Crystallin promoter

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August undefined, 2024

WebApr 8, 2024 · Among the 14 crystallin genes that human and mouse have in common, mRNA levels of Crybb1, Crygd, Cryba2, Cryba4, Crybb3, Cryga, and Crygs were significantly up-regulated in myopic eyes of... WebThe crystallin genes are expressed at relatively low levels in many other tissues besides lens, where they apparently function in various ways in stress amelioration; thus for example, the α-crystallin family of lens proteins is similar to the small heat shock proteins, and one of these genes responds directly to a xenobiotic response factor …

The human crystallin gene families - PMC - National Center for ...

WebCrystallin promoters of widely different species are dependent on the same suite of transcription factors and function in similar ways in lens-cells of other species both in culture and in transgenic models (for example: [31–33]). This allows comparison of promoters from both chicken and mouse in mouse cells. WebMar 1, 2003 · In order to study lens-retina relationships during development, we cloned the zebrafish alphaA-crystallin cDNA and its promoter region. Using a 2.8-kb fragment of the zebrafish alphaA-crystallin promoter (z (alpha)Acry), we expressed the diphtheria toxin A fragment (DTA) in zebrafish embryos in a lens-specific manner. cypher setups for every map

Perturbed development of the mouse lens by polyomavirus large …

WebJul 5, 2002 · Crystallin promoter reporters. The constructs with the αA-, βB2-, and γF-crystallin promoters linked to a luciferase reporter are depicted. Potential c-Maf recognition sites are located at −110 to −98 in the αA and βB2 promoters, and at −47 to −35 in the γF promoter ( 30, 31, 32 ). WebBy use of a gene fusion of the alpha A-crystallin promoter to the viral immortalizing oncogene, polyoma large T antigen (PyLT), we created transgenic mice that express PyLT specifically in ocular lens. Expression of large T antigen during embryonic development led to a perturbation in lens development, specifically, an interference with the ... WebDec 1, 2012 · In many crystallin gene promoters, elements for lens and non-lens expression are combined in the same 5 ′ region. However, one of the more recently … cypher setups all maps

Gamma Crystallin - an overview ScienceDirect Topics

Transcriptional Regulation of the Mouse αA-Crystallin Gene: …

WebA DCR1/αA-crystallin promoter (including DCR2) coupled with EGFP virtually recapitulated the expression pattern of αA-crystallin in lens epithelium and fibers. In contrast, the DCR3/αA/EGFP reporter was expressed only in 'late' lens fibers. Chromatin immunoprecipitations showed binding of Pax6 to DCR1 and the αA-crystallin promoter … WebI am a highly motivated and passionate individual with a talent for organization and project management. I have enjoyed leading teams to complete projects in various industries … cypher setups on fractureWebSep 1, 2002 · These data suggest that the chicken β B1-crystallin promoter is the promoter of choice to direct the high level ectopic expression of RNAs to lens fiber cells … cypher setups on ascent

"WebMay 4, 2006 · Dramatic upregulation of αA-crystallin expression is associated with primary lens fiber cell differentiation. Previous studies have shown that a −366 to +46 αA … " - Crystallin promoter

Crystallin promoter

Regulation of Expression within a Gene Family

WebPromoter methylation of RASEF was associated with increased risk of death due to metastasis, ... Epigenetic regulation of αA-crystallin in high myopia-induced dark nuclear cataract. PLoS One 2013; 8: e81900. doi: 10.1371/journal.pone.0081900. 8. Mao W, Rubin JS, Anoruo N, Wordinger RJ, Clark AF. WebLens-specific expression and developmental regulation of the bacterial chloramphenicol acetyltransferase gene driven by the murine alpha A-crystallin promoter in transgenic …

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WebThis study concluded that there existed a TGF-β responsive element in the promoter just 285 base pairs upstream of the transcriptional start site ... porcine TGF-β1 under the lens-specific αA-crystallin promoter and then another similar line was created but with the gene driven by the βb-crystallin promoter, corresponding to another lens ... WebSep 1, 2002 · Thus, the chicken betaB1-crystallin promoter directs high level RNA expression to lens fiber cells, which may be especially useful for the expression of ribozyme and anti-sense RNAs in addition to ...

WebA conserved and essential DNA motif in the promoters of the γ-crystallin genes is the binding site of the SOX proteins that activate the promoters (Kamachi et al., 1995). … WebWe have further compared the γD-crystallin promoter with the γB-crystallin promoter, the promoter with the most extended developmental expression. We show that the γ …

WebEarlier experiments showed that the betaB1-crystallin promoter sequence from -120 to -48 contains a cis element (PL2 at -90 to -76) that stimulates the activity of a heterologous promoter in lens cells but not in fibroblasts. WebThe human papillomavirus type 16 (HPV-16) E6 and E7 oncogenes are thought to play a role in the development of most human cervical cancers. These E6 and E7 oncoproteins affect cell growth control at least in part through their association with and

WebCrystallins are a diverse group of abundant soluble proteins that are responsible for the refractive properties of the transparent eye lens. We showed previously that Pax-6 can activate the αB-crystallin/small heat shock protein promoter via the lens-specific regulatory regions LSR1 (−147/−118) and LSR2 (−78/−46).

WebProx1 activates several of the different crystallin promoters, and acts synergistically with another lens transcription factor, Maf, to activate transcription of ß1 and ß2 crystallins (Lengler ... cypher setup on havenWebSox-1 transactivated the γF-crystallin promoter in a dose-dependent fashion, similar to c-Maf (Fig. 2 C). Sox-1 also stimulated the βB2 promoter, but less strongly than c-Maf (Fig. 2 B). Prox-1 (80 ng) stimulated the βB2- and γF-crystallin promoters only about 2-fold (Fig. 2, B and C). To determine whether these transcription factors can ... cypher setups on iceboxWebThe same SOX proteins also regulate γ-crystallin genes. the mammalian counterpart of δ crystallin. A conserved and essential DNA motif in the promoters of the γ-crystallin genes is the binding site of the SOX proteins that activate the promoters (Kamachi et al., 1995). SOX1 strongly, and SOX2 and SOX3 modestly, activate these crystallin ... cypher setups on pearlWebThe two paralogous zebrafish αB-crystallin promoters produced subtly different expression profiles, with the aBa promoter driving expression equally in notochord and skeletal muscle while the αBb promoter resulted primarily in skeletal muscle expression. cypher setups on breezeWebDec 3, 2013 · CpG island methylation in the αA-crystallin promoter was similar in age-related cataract and high-myopic cataract classified as Lens Opacity Classification System III Grade Nuclear Color 2–3. Pyrosequencing, a real-time DNA sequencing method, was used to determine single-base variations caused by CpG methylation of the CRYAA … cypher setup lotusWebJul 15, 1992 · Progeny from two of these lines were mated with transgenic mice expressing the Cre recombinase under control of either the murine alpha A-crystallin promoter or … cypher set up splitWebMar 30, 2024 · Stimulation of αA-crystallin promoter activity via 8-bromo-cAMP, forskolin, or human T-cell leukemia virus type I Tax1 in transfections and reduction of activity of this site in cell-free transcription tests by competition with the somatostatin CRE supported the idea that DE1 is a functional CRE. Finally, Pax-6, a member of the paired-box ... cypher setup split