Cst atf4 antibody

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August undefined, 2024

WebNov 1, 2024 · Membranes were then incubated at 4 °C overnight with ATF4 antibody (BioLegend) at 1:1000. After washing with 1x TBST, anti-rat HRP secondary antibody (CST) was used at 1:2000. A list of commercially available antibodies tested in livers and cell lines is included in Supplementary Table 1. 2.6. Gene expression analysis. WebHowever, CS-11815 detects a band with a mass of ~49 kDa, while the expected molecular weight of ATF4 is ~38 kDa. In contrast, SC-200 is a very dirty polyclonal antibody, but it seems to detect ...

ATF4 selectively regulates heat nociception and contributes to kines…

Web(11815) ATF-4 (D4B8) Rabbit mAb - Cell Signaling Technology - CiteAb. 670 citations – A rabbit monoclonal antibody, raised against Cyclic AMP-dependent transcription factor ATF-4 (Human), supplied by Cell Signaling Technology. ... ATF4, Activating transcription factor 4, CREB-2, CREB2, Cyclic AMP-dependent transcription factor ATF-4, Cyclic ... WebMay 5, 2024 · About 500 mg protein of total cell lysates was incubated with primary antibody anti-ATF4 (#11815, CST or 10835-1-ap, Proteintech group, USA) or anti-CTCF (#3418, CST or sc271514, Santa Cruz Biotechnology, USA) at 4 °C with overnight shaking. The immune complexes were then incubated with Protein A/G Dynabeads (Thermo … how much sodium in sodium chloride 0.9%

CHST4 Gene - GeneCards CHST4 Protein CHST4 Antibody

WebJun 24, 2024 · PERK IPs were performed with 1 mg of lysate incubated with 2 µg of primary antibody (Proteintech, #20582-1AP) or the corresponding IgG (Cell Signaling Technology, #2729) overnight at 4 °C with ... WebDec 19, 2024 · Shipping Temperature : Blue Ice or 4ºC. Cite this Product : StressMarq Biosciences Cat# SMC-447D, RRID: AB_2701793. Certificate of Analysis : 1 µg/ml of SMC-447 was sufficient for detection of ATF4 in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. WebResearch studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the … how much sodium in sodium citrate

ATF4 selectively regulates heat nociception and contributes to …

The mTORC1-mediated activation of ATF4 promotes …

WebAug 13, 2024 · This was especially relevant as we found in the public CHIP-seq data base (chip-atlas.org), that the Nrf2 antibody bound to the ATF4 promoter (~1 kB from the TSS) in the cardiovascular HAEC cell ... WebSep 5, 2024 · ATF4 is induced in CTLs exposed to tumor-derived factors and antigen stimulation. We sought to determine the expression of ATF4 in CD8 + T cells upon their activation or exposure to the stress stimuli and tumor-derived factors. Treatment of mouse splenic CD8 + T cells with thapsigargin, an inducer of the endoplasmic reticulum stress … how do we add fractions how much sodium in sodium phosphate

"WebMar 3, 2024 · In the nervous system, activating transcription factor 4 (ATF4) is involved in the regulation of synaptic plasticity and memory formation. Here, we show that ATF4 … " - Cst atf4 antibody

Cst atf4 antibody

CREB-2/ATF-4 Antibody (B-3) SCBT - Santa Cruz Biotechnology

WebOur ATF4 polyclonal, monoclonal and recombinant monoclonal antibodies are developed in Rabbit, Mouse and Goat. These antibodies have been verified by Cell treatment, … WebJun 14, 2013 · The transcription factor activating transcription factor 4 (ATF4) belongs to the cAMP-responsive element–binding protein family, characterized by the presence of a leucine zipper dimerization domain and a basic amino acid–rich DNA binding domain ().Previous studies have shown that knocking out ATF4 gene expression in mice results …

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WebSep 29, 2024 · ATF-4 Antibody (B-3) is an IgG 2b κ mouse monoclonal ATF-4 antibody (also designated ATF4 antibody, cAMP-dependent transcription factor ATF-4 antibody, CREB-2 antibody or cAMP … WebActivating transcription factor 4 antibody; ATF 4 antibody; ATF4 antibody; ATF4 protein antibody; ATF4_HUMAN antibody; cAMP-dependent transcription factor ATF-4 antibody; cAMP-responsive element-binding …

WebFeb 17, 2024 · Autophagy is a cellular process through which cells engulf and degrade damaged cytoplasmic components ().Recent studies have implicated the involvement of autophagy in the regulation of obesity and energy expenditure (15–21).For example, autophagy is reduced in the hypothalamus of aged mice or mice fed an HFD … WebATF4 Rabbit pAb (A0201) Western blot analysis of extracts of HeLa cells, using ATF4 antibody (A0201) at 1:1000 dilution.HeLa cells were treated by tunicamycin (2 μg/mL) for 8 hours. Secondary antibody: HRP Goat Anti …

WebMonoclonal Antibody for studying ATF-4. Cited in 661 publications. Validated for WB, IP, IF, ChIP, ChIP, C&R. Highly specific and rigorously … WebThis antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated …

WebDec 25, 2024 · The membrane was blocked and then incubated overnight at 4°C with primary antibody as described previously ... antibody to phospho-T389 p70S6K1 was obtained from Cell Signaling Technology (catalog number: 9205); antibodies to REDD1 (catalog number: 10638-1-AP), Sestrin1 (catalog number: 21668-1-AP), and Sestrin2 …

WebMar 25, 2024 · Some oncoproteins along with stress kinase general control non-derepressible 2 (GCN2) can ensure the induction of activating transcription factor 4 (ATF4) to counteract amino acid deprivation; however, little is known regarding the role of the oncogenic EGFR-PI3K pathway. In this study, we demonstrate that both mutated EGFR … how do we assess circulationWebOct 20, 2024 · ChIP assays with NFATc1 or ATF4 antibody demonstrated that isoproterenol increased NFATc1 and ATF4 binding to their cognate binding elements in RANKL ... MA, USA). CREB1 and phospho-CREB1 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA), and phospho-ATF4 antibody was … how much sodium in spicesWebrepresentative of CST, are rejected and are of no force or effect. P roduc t s are labeled wit h For Res earc h Us e O nly or a s imilar labeling s t at ement and hav e not been approv ed, c leared, or lic ens ed by t he FDA or ot her regulat ory f oreign or domes t … how much sodium in soft pretzelWebOct 22, 2010 · ATF4 is a transcriptional activator of the integrated stress response, a program of gene expression involved in metabolism, nutrient uptake, anti-oxidation, and … how do we as humans feel.lovedWebCST4. Cystatin-S is a protein that in humans is encoded by the CST4 gene. [3] [4] The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. … how do we arrive at godWebJan 10, 2024 · Moreover, ATF4 overexpression inhibited tumor proliferation and AR expression both in vitro and in vivo. Collectively, these results demonstrated that ER … how do we ask for forgiveness from godWebMonoclonal Antibody for studying ATF-4. Cited in 3 publications. Validated for Western Blotting, Immunofluorescence (Immunocytochemistry). Highly specific and rigorously … how much sodium in sodium phos