Destabilization of medial meniscus
WebAug 12, 2013 · 1. Make an incision of the skin on the medial side of patellar ligament using a #15 blade. 2. Fold back the skin and locate the patellar ligament (Figure 1). 3. Make an … WebFeb 12, 2024 · The DMM (destabilization of the medial meniscus) model is now the most commonly used surgical model in mice. Its reliability, reproducibility, structural similarity to human OA, its relatively...
Destabilization of medial meniscus
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WebThis chapter describes the surgical procedure for destabilization of medial meniscus in mice. Details on subsequent microCT and histological analysis are also provided, as well as details on osteoarthritis evaluation. Related articles Based on techniques Generation and Characterization of Mouse Models for Skeletal Disease WebThe medial meniscus is a fibrocartilage semicircular band that spans the knee joint medially, located between the medial condyle of the femur and the medial condyle of the tibia. [1] It is also referred to as the internal …
WebAnimals (mice or rats) are anesthetized with Isoflurane and the right knee area and lower leg are prepared for surgery. For the surgical destabilization of the medial meniscus (DMM), a skin incision is made from the distal patella to the proximal tibial plateau. The joint capsule immediately medial to the patellar tendon is incised and spread ... WebMay 1, 2024 · Conclusions. We developed medial meniscus extrusion in a rat model, and centralization of the extruded medial meniscus by the pull-out suture technique improved the medial meniscus extrusion and delayed cartilage degeneration, though the effect was limited. Centralization is a promising treatment to prevent the progression of osteoarthritis.
WebMar 22, 2024 · 22 Mar 2024 All issues ONLINE COVER Mind the gp130. In this image of a mouse knee joint after medial meniscus destabilization, Safranin O staining reveals proteoglycan-rich cartilage (pink) at the joint surface, suggesting resistance to osteoarthritis. WebOsteoarthritis Mouse Model of Destabilization of the Medial Meniscus This chapter describes the surgical procedure for destabilization of medial meniscus in mice. Details on subsequent microCT and histological analysis are also provided, as well as details on osteoarthritis evaluation.
WebDestabilization of medial meniscus (DMM) model is an important tool for studying the pathophysiological roles of numerous arthritis associated molecules in the pathogenesis of osteoarthritis (OA) in vivo. However, the detailed, especially the visualized protocol for establishing this complicated model in mice, is not available.
WebApr 10, 2024 · In this study, a destabilization of the medial meniscus (DMM)-induced OA model was constructed, and SDF-1α expression in the OA model was evaluated. The CXCR4 receptor antagonist AMD3100 was used to block SDF-1α, and changes in subchondral bone and the expression of BMSCs in subchondral bone in the OA model … someone else logged into my facebookWebApr 7, 2024 · 按文献方法采用内侧半月板失稳(destabilization of medial meniscus,DMM)手术诱导建立KOA模型[9],简述如下:右膝关节内侧髌韧带与内侧副韧带之间开0.5 cm切口,显微镜下暴露、剪断内侧半月板胫骨韧带,缝合切口。 small business sustainability policy templatehttp://jur.byu.edu/?p=1626 small business support program tasWebJan 1, 2014 · The surgical model of destabilization of the medial meniscus (DMM) has become a gold standard for studying the onset and progression of posttraumatic osteoarthritis (OA). The DMM model … someone else saywecanfly lyricsWebSep 29, 2016 · Then we induced OA in rats through surgical destabilization of the medial meniscus (DMM), and evaluated the effects of AMD3100 on the progression of OA and aggrecan metabolism. Third, we cultured rat primary chondrocytes with untreated control, CXCL12a, siNC + CXCL12a, or siRNA CXCR4 + CXCL12a and confirmed ACAN, SOX … small business svgWebApr 1, 2024 · Methods Destabilization of the medial meniscus (DMM) or sham surgeries were performed on 10-week-old male wild-type (WT) and Camkk2-/- mice. Half of the DMM-WT mice and all other cohorts ... someone else showed up on my background checkWebFeb 9, 2024 · Age is the primary risk factor for osteoarthritis (OA), yet surgical OA mouse models such as destabilization of the medial meniscus (DMM) used for evaluating disease-modifying OA targets are ... small business svg free