WebDepends on what kind of data your plotting. If it is binding, is it one or two site competition? Also, you need to make sure the X-axis drug concentrations are labelled properly.

The GraphPad Guide to Analyzing Radioligand Binding Data

WebCompetition Binding Assays. Competition binding assays (total volume 100 μl) were performed using a final assay concentration of 50 nM, 100 nM, or 1 µM [3 H] (+) pentazocine with increasing concentrations of DTG (10 nM–1 mM). Alternatively, 10–30 nM [³H] DTG was employed in the presence of increasing concentrations of (+) pentazocine (10 ... WebWhat is competitive binding? In a competitive binding experiment, you use a single concentration of labeled (hot) ligand and vary the concentration of unlabeled (cold) … green night light for hunting

Receptor Binding Assays for HTS and Drug Discovery

WebGraphPad Prism 9 Curve Fitting Guide - Receptor binding - Competitive binding. In a competitive binding experiment, you use a single concentration of labeled (hot) ligand … WebSaturation Binding Data graph contains the points from the saturation binding experiment, and the table labeled Scatchard Y and X intercepts contains two points marking the Y … WebThe equation below will be used to compute the Ki value of the given inhibitor: The above equation shows that Ki can be expressed as a function of the concentration of the free inhibitor at 50% inhibition, [ I] 50, the concentration of the free labeled ligand at 50% inhibition, [ L] 50, the concentration of the free protein at 0% inhibition ... fly like django twitch