Smad3 western blot

WebbWestern Blot (WB) 1:500-1:2000 - Visit thermofisher.com to view references and figures. Products are warranted to operate or perform substantially in conformance with published Product specifications in effect at the time of sale, as set forth in the Production documentation, specifications and/or accompanying package inserts ("Documentation"). WebbSMAD3 Antibodies. Antibodies that detect SMAD3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, …

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WebbBiorbyt's SMAD3 antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: Immunohistochemistry, and Western Blot. The SMAD3 … Webb11 okt. 2024 · S1 Fig: SMAD3 promotes expression of differentiation markers, migration, and inhibits proliferation of HCASMC. A) HCASMC were treated with SMAD3-specific (S3 KD) or scrambled sequence (SCR) siRNA, and evaluated by western blot analysis for expression of lineage markers ACTA2 and TAGLN and control protein GAPDH. Identical … how to see logs in application insights https://urlinkz.net

Anti-SMAD3 Antibodies Invitrogen - Thermo Fisher Scientific

WebbWestern blotting was performed to detect the expression of Mstn, phosphatidylinositol 3-kinase (PI3K), protein kinase B (Akt), small ribonucleoprotein particle protein 2 (Smd2), small ribonucleoprotein particle protein 3 (Smd3), and small ribonucleoprotein particle protein 4 (Smd4). Webb10 juli 2012 · The expressions of TGF-β1/Smad signaling-related genes including type I and III collagen in human and rat HSCs before and after 18α-GA treatment were measured by real time PCR. The expression of related proteins was verified by western blot assay. The phosphorylation level of Smad2 and Smad3 was detected by immunocytochemistry. WebbThe Total-SMAD3 assay quantifies the expression level of SMAD3 in a cell lysate. Contrary to Western Blot, the assay is entirely plate-based and does not require gels, … how to see logs in eclipse

Figure 6 Western blot analysis of Smad3 expression and its...

Category:Smad3 Antibody - Cell Signaling Technology

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Smad3 western blot

Smad2 and Smad3 have differential sensitivity in relaying TGFβ ...

WebbCurcumin treatment significantly inhibited the expressions of smad3, α-SMA, COL1A1, COL3A1, as well as miR-21 expression in NRK-49F cells in a dose-dependent manner.ConclusionCurcumin exerted its anti-fibrotic effects by targeting the TGF-β1/smad3 signaling pathway and suppressing miR-21 expression, thereby provides novel … WebbWestern Blot (WB) 1/500 - 1/2000 3 Publications Miscellaneous Pubmed (Misc) - 1 Publication Product Specific Information MA5-15663 targets SMAD3 in ICC, IF and WB applications and shows reactivity with Human and mouse samples. The MA5-15663 immunogen is purified recombinant fragment of human SMAD3 expressed in E. Coli..

Smad3 western blot

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Webbcells and Th17 cells. The specificity of the O72-670 mAb was confirmed by Western blot and immunohistochemistry using unconjugated antibody. The BD Horizon™ Red 718 (R718) Dye is part of the BD red family of dyes. It is a small organic fluorochrome with an excitation maximum (Ex Max) at 695-nm and an emission maximum (Em Max) at 718-nm. WebbHuman Phospho-Smad2/3 (S465/S467) Antibody Summary Species Reactivity Human Specificity Detects human Smad2 and Smad3 when dually phosphorylated at S465 and …

Webb4 apr. 2024 · Methods and Results: Western blot data showed that APE1 and SMAD3 were highly expressed in EAC cell lines. APE1 silencing reduced SMAD3 nuclear expression … WebbAll lanes : Anti-Smad3 (phospho S213) antibody (ab63403) at 1/500 dilution Lane 1 : Extracts from HT29 cells Lane 2 : Extracts from HT29 cells plus phospho peptide …

Webb5 feb. 2024 · Total protein samples were extracted from the cells and equal amounts of total protein (20 μg) were subjected to Western blotting analysis. The levels of indicated proteins were assessed by a standard immuno-blotting technique with a specific primary antibody. Actin indicates equal amounts of protein loading into each lane (left panel). WebbThis study was to observe effects of puerarin on expression of cardiac transforming growth factor β1 (TGF-β1), Smad3 and Smad7 mRNA in the spontaneously hypertensive rat (SHR), and to explore its possible mechanism of myocardial protection.Materials and methodsThirty-five 12-week-old SHRs were randomly allocated into 5 groups of 7 rats as …

WebbSMAD3 antibody (pAb) tested by Western blot. Whole cell extract of A549 cells (30 µg) probed with SMAD3 antibody (1:500). Background. This SMAD3 (mothers against …

Webb摘要： 目的 探讨血管紧张素转化酶2 (ACE2)对缺氧复氧诱导的肾小管上皮细胞HK-2氧化应激、炎症、凋亡及核因子E2相关因子2 (Nrf2)/血红素加氧酶1 (HO-1)信号通路的影响。. 方法 将ACE2慢病毒转染HK-2细胞,按照实验需要分为常氧组 (Control组)、缺氧复氧模型组 (H/R组 … how to see logs in linuxWebbWestern blot是评估蛋白磷酸化状态的最常用方法，利用SDS-PAGE分离蛋白样品，随后转移到印迹膜上，之后利用磷酸化特异性抗体来鉴定目的蛋白。. 有人会说western blot还不 … how to see logs of pod kubectlhttp://yxbwk.njournal.sdu.edu.cn/CN/10.6040/j.issn.1671-7554.0.2024.1226 how to see logs in puttyWebb16 aug. 2024 · This affinity purified antibody has been tested for use in ELISA, immunohistochemistry, and western blot. Specific conditions for reactivity should be … how to see logs in tpotWebbWestern blot assay The purified cells treated with MENK and untreated cells were lysed in cell disruption buffer (mirVana TMPARIS RNA and Native Protein Purification Kit; Invitrogen Life Technology, CA). Protein samples (40 mg) were subjected to electrophoretic analysis through 10% SDS-PAGE43 and subsequently transferred how to see logs in splunkWebbSMAD3 抗体 SMAD3 抗体（anti SMAD3 antibody）は、SMAD3 ... Various cells were subjected to SDS PAGE followed by western blot with 66516-1-Ig (SMAD3 antibody) at … how to see london in 3 daysWebbFree shipping on catalog antibody and protein orders over $999. More>> how to see logs in windows 11